At Synbio Technologies, we are committed to providing customized CRISPR sgRNA library construction services. Our delivered libraries guarantee:
✔ Coverage > 99%
✔ Uniformity (CV) < 5-10
✔ Accuracy > 80%

High-quality libraries empower clients to achieve breakthroughs in gene function research, drug target screening, and more.


Leveraging our high-throughput design and synthesis platform, Synbio Technologies successfully constructed a high-coverage, highly uniform canine whole-genome CRISPR/Cas9-sgRNA plasmid library. This library is instrumental in studying canine virus replication mechanisms and screening potential antiviral drug targets.

• High-Throughput Design & Synthesis: Designed and synthesized 3-4 sgRNAs per gene efficiently.
• Efficient Cloning: Utilized BsmB I sites to clone sgRNA into the lentiCRISPR V2 , ensuring library diversity and uniformity.
• Library Validation: High-throughput sequencing confirmed 99.96% coverage and excellent uniformity. 



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Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA